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通过体系紫外吸收和荧光强度的变化,研究了饮用水消毒副产物三溴甲烷(CHBr3)、三氯甲烷(CHCl3)与牛血清蛋白(BSA)之间的相互作用.结果表明,CHBr3和CHCl3均对BSA的内源荧光具有静态猝灭作用,其中CHBr3与BSA的结合强于CHCl3.Fe3+,Al3+和聚丙烯酰胺的加入也使BSA的荧光强度降低,与BSA的结合的强弱依次为:聚丙烯酰胺>Fe3+>Al3+.在二元体系的基础上,进一步研究了Fe3+,Al3+和聚丙烯酰胺对BSA-CHBr3(CHCl3)结合作用的影响.结果表明,Fe3+,Al3+和聚丙烯酰胺的存在使BSA-CHCl3、BSA-CHBr3的结合常数呈现降低的趋势,降低的趋势为Al3+>聚丙烯酰胺>Fe3+.
The interaction between tribromomethane (CHBr3), trichloromethane (CHCl3) and bovine serum albumin (BSA), a by-product of drinking water disinfection, was studied by the changes of UV absorption and fluorescence intensity.The results showed that both CHBr3 and CHCl3 were The endogenous fluorescence of BSA has a static quenching effect, in which the binding of CHBr3 to BSA is stronger than that of CHCl3.Fe3 +, Al3 + and polyacrylamide also decrease the fluorescence intensity of BSA, the binding strength to BSA is: polypropylene Amide> Fe3 +> Al3 + .The influence of Fe3 +, Al3 + and polyacrylamide on the binding of BSA-CHBr3 (CHCl3) was further studied on the basis of binary system.The results showed that the presence of Fe3 +, Al3 + The binding constants of -CHCl3 and BSA-CHBr3 showed a decreasing trend, and the decreasing tendency was Al3 +> polyacrylamide> Fe3 +.