论文部分内容阅读
原位杂交和免疫组织化学是当前生物化学和分子技术进行基因及产物研究的常用方法 ,可确定各个细胞中mRNA和蛋白质在何时何处表达。这两项细胞技术的成功与否 ,关键在于载玻片的正确处理。经典的载玻片处理技术用加有洗涤剂的水清洗载玻片数分钟后 ,再于水中浸泡 30min。
In situ hybridization and immunohistochemistry are common methods currently used in biochemistry and molecular techniques for gene and product studies to determine when and where mRNAs and proteins are expressed in individual cells. The key to the success of these two cell technologies is the correct handling of slides. Classic slide handling techniques Wash the slide with detergent-added water for a few minutes and then soak in water for 30 minutes.