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[Objective] To establish an analytical method for simultaneously determining the alisol C 23-acetate,Alisol A,alisol B,alisol B23-acetate in RHIZOMA ALISMATIS. [Methods]The assay was performed on a Ultimate XB- C18column(4. 6 mm × 250 mm,5 μm) with the mobile phase consisting of acetonitrile- water(65∶35) at a flow rate of 1. 0 mL /min. The column temperature was at 30 ℃ and the optimum detection wavelength of DAD was 245 nm. The drift tube and atomizer temperatures of ELSD were 60 and 55 ℃,respectively,with a carrier gas(N2) pressure of 20 psi. [Results]The linear range of alisol C 23-acetate,Alisol A,alisol B and alisol B 23-acetate were 1.869-29. 90 μg /mL(r = 0. 999 8),3. 731- 74. 62 μg /mL(r = 0. 999 7),8. 653- 138. 4 μg /mL(r = 0. 999 9),and 4. 832- 77. 31 μg /mL(r =0. 999 5),respectively. The average recovery rates(n = 6) were 98. 78%(RSD = 2. 63%),98. 05%(RSD = 2. 72%),98. 26%(RSD =2. 86%) and 97. 65%( RSD = 2. 95%),respectively. 20 batches of RHIZOMA ALISMATIS were detected by this method; and results showed that there were relatively great differences in the contents of four triterpenoids between RHIZOMA ALISMATIS grown in Sichuan and Fujian. The average content of total triterpenoids in 10 batches of RHIZOMA ALISMATIS grown in Sichuan was significantly higher than that grown in Fujian. The content of former was 2. 499- 4. 701 mg /g; while the content of latter was 1. 210- 3. 523 mg /g. Besides,alisol A was not detected in the RHIZOMA ALISMATIS grown in Fujian Province; while the alisol A content in RHIZOMA ALISMATIS grown in Sichuan was relatively high. Therefore,this feature could be used for the identification of two RHIZOMA ALISMATIS. [Conclusions] The HPLCDAD-ELSD quantitative analysis method was simple,rapid and accurate,and could be used for the quantitative analysis of multi-component of RHIZOMA ALISMATIS,which provided a novel approach for the comprehensively evaluation of the quality of RHIZOMA ALISMATIS.
[Objective] To establish an analytical method for simultaneously determining the alisol C 23-acetate, Alisol A, alisol B, alisol B23-acetate in RHIZOMA ALISMATIS. [Methods] The assay was performed on a Ultimate XB-C18column × 250 mm, 5 μm) with the mobile phase consisting of acetonitrile-water (65:35) at a flow rate of 1.0 mL / min. The column temperature was at 30 ° C and the optimum detection wavelength of DAD was 245 nm . The drift tube and atomizer temperatures of ELSD were 60 and 55 ° C., respectively, with a carrier gas (N2) pressure of 20 psi. [Results] The linear range of alisol C 23-acetate, Alisol A, alisol B and alisol B 23-acetate were 1.869-29 90 μg / mL (r = 0. 999 8), 3. 731-74.62 μg / mL (r = 0.999 7), 8.653-138.4 μg / mL (r = 0.999 9), and 4. 832-77.31 μg / mL (r = 0.9999), respectively. The average recovery rates (n = 6) were 98. 78% (RSD = (RSD = 2.72%), 98.26% (RSD = 2.86%) and 97.65% (RSD = 2.95%), respectively. 20 batches of RHIZOMA ALISMATIS were detected by this method; and results showed that there were relatively great differences in the contents of four triterpenoids between RHIZOMA ALISMATIS grown in Sichuan and Fujian. The average content of total triterpenoids in 10 batches of RHIZOMA ALISMATIS grown in Sichuan was significantly higher than that grown in Fujian. The content of former was 2. 499- 4. 701 mg / g; while the content of latter was 1. 210-3. 523 mg / g. Besides, alisol A was not detected in the RHIZOMA ALISMATIS grown in Fujian Province, while the alisol A content in RHIZOMA ALISMATIS grown in Sichuan was relatively high. Thus, this feature could be used for the identification of two RHIZOMA ALISMATIS. [Conclusions] The HPLCDAD-ELSD quantitative analysis method was simple, rapid and accurate, and could be used for the quantitative analysis of multi-component of RHIZOMA ALISMATIS, which provided a novel approach for the comprehensively evaluation of the quality of RHIZOMA ALISMATIS.