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试验采用高效液相色谱法比较不同来源酒花浸膏对异合葎草酮(Humulone)、异葎草酮(Isohumulone)、异加葎草酮(Tetrahydroisoαacid)、合葎草酮(Cohumulone)、葎草酮(Humulone)、加葎草酮(Adhumulone)、合蛇麻酮(Colupulone)、蛇麻酮(Lupulone)及加蛇麻酮(Adlupulone)9种味觉物质含量的影响。试验采用Waters YMCTMCarotenoid 3μm色谱柱(4.6 mm×150 mm),流动相A相为0.1%H_3PO_4(含0.2 mmol/L EDTA);流动相B相为乙腈,梯度洗脱,流速为1.0 m L/min,DAD检测波长为315 nm;柱温为40℃。检测方法显示:9种味觉物质的检测线性范围为0.01~500 mg/L;方法的检出限在0.02~0.2 mg/kg之间,在10~500μg/g的加标条件下的加标回收率在97.48%~105.12%之间,精密度为1.25%~3.02%(n=6),检测方法灵敏、有效。检测结果表明,不同来源酒花浸膏得到的酒花浸膏中异合葎草酮、异葎草酮、异加葎草酮、合葎草酮、葎草酮、加葎草酮、合蛇麻酮、蛇麻酮及加蛇麻酮9种酒花浸膏味觉物质的总含量以青岛大花酒花最高。
The effects of different extracts of hop extracts on the metabolism of Humulone, Isohumulone, Tetrahydroisoαacid, Cohumulone, Humulone, Adhumulone, Colupulone, Lupulone and Adlupulone. The mobile phase A was 0.1% H_3PO_4 (containing 0.2 mmol / L EDTA), and the mobile phase B was acetonitrile with gradient elution at a flow rate of 1.0 mL / min using a Waters YMCT Carotenoid 3 μm column (4.6 mm × 150 mm) , DAD detection wavelength was 315 nm; column temperature was 40 ℃. The detection methods showed that the linear range of detection of nine kinds of taste substances was from 0.01 to 500 mg / L and the detection limit of the method was within the range of 0.02 to 0.2 mg / kg, spiked under the spiked condition of 10 ~ 500 μg / g Rates ranged from 97.48% to 105.12%, and the precision was 1.25% to 3.02% (n = 6). The detection method was sensitive and effective. The results showed that the different sources of hops extract hop extract obtained isohumulone, isohumulone, isohumulone, haulmone, humulone, humulone, , Lupulone and add hop hop ketone nine kinds of hops extracts taste the total content of Qingdao flower Hops the highest.