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采用点滴法,于室内测定了阿维菌素、茚虫威和鱼藤酮3种杀虫剂单独使用及与绿僵菌混用对沙葱萤叶甲3龄幼虫的协同致死作用,以期为协调使用化学药剂和生防制剂防治沙葱萤叶甲提供参考。3种杀虫剂单独使用时的毒力测定结果表明,阿维菌素对沙葱萤叶甲3龄幼虫的毒力最强,LD50值为4.80 ng/头,其次是茚虫威和鱼藤酮,LD50值分别为10.47和53.21 ng/头。采用不同浓度的3种杀虫剂分别处理绿僵菌24和48 h后,发现药剂对绿僵菌分生孢子萌发均有一定的抑制作用,但抑制率低于20%,且抑制率随着药剂浓度的降低和作用时间的延长而降低,其中茚虫威的平均抑制率最小,分别仅为1.89%±0.57%和0.89%±0.39%。将3种杀虫剂分别与绿僵菌孢子悬液混配使用,对防治沙葱萤叶甲3龄幼虫均具有协同增效作用:其中茚虫威与绿僵菌混用增效作用最强,比单独施用绿僵菌时LT50值缩短了8.12 d;阿维菌素与绿僵菌混用时LT50值缩短了7.60 d;鱼藤酮与绿僵菌混用时LT50值缩短了6.45 d。
The synergistic lethal effect of avermectin, indoxacarb and rotenone on the 3rd instar larvae of A. annua was studied by using the method of single-drop method in combination with Metarhizium anisopliae in laboratory, Pharmacy and biocontrol agents for control Shade Diaojia provide a reference. The virulence of the three pesticides when used alone showed that avermectin had the strongest virulence to the 3rd instar larvae of Diabrotica philippinensis with an LD50 value of 4.80 ng / head, followed by indoxacarb and rotenone, LD50 values were 10.47 and 53.21 ng / head, respectively. Metarhizium anisopliae was treated with three insecticides at different concentrations for 24 and 48 h, respectively. It was found that the agents inhibited the spore germination of Metarhizium anisopliae to some extent, but the inhibitory rate was less than 20% The concentration of indoxacarb was the lowest, which was only 1.89% ± 0.57% and 0.89% ± 0.39%, respectively. The three insecticides were mixed with the spores suspension of Metarhizium anisopliae, respectively, to control the third-instar larvae of Diabrotica trifoliatus have Synergistic effect: Indoxacarb and Metarhizium mixed the strongest synergistic effect, Compared with the single application of Metarhizium, the LT50 value was shortened by 8.12 days. The LT50 value was shortened by 7.60 days when the avermectin was mixed with Metarhizium anisopliae. The LT50 value was shortened by 6.45 days when rotenone was mixed with Metarhizium anisopliae.