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To evaluate the role of salidroside on proliferation,apoptosis and invasiveness of salivary gland adenoid cystic carcinoma cells(SACC),immunocytochemical staining was employed to detect proliferating cell nuclear antigen(PCNA),caspase 3 and caspase 8 expression in SACC-2 cells.Modified Boyden chamber assay combined with laser confocal microscopy(LSCM) was used to evaluate the invasion and migration abilities of SACC-2 cells at different time point.Immunohistochemistry staining revealed that the expression of PCNA was significantly decreased(P<0.01) after salidroside treatment.In contrast,salidroside treatment led to increased caspase 3 and caspase 8 in SACC-2 cells.Cell migration depth and number of cells that penetrated Boyden chamber were also decreased by salidroside.Salidroside potently inhibits the proliferation and simultaneously induces the apoptosis of SACC-2 cells.Migration and invasion of SACC-2 cells are also inhibited.Our data throw light on potential clinical application of salidroside to the patients with SACC.
To evaluate the role of salidroside on proliferation, apoptosis and invasiveness of salivary gland adenoid cystic carcinoma cells (SACC), immunocytochemical staining was employed to detect proliferating cell nuclear antigen (PCNA), caspase 3 and caspase 8 expression in SACC-2 cells. Modified Boyden chamber assay combined with laser confocal microscopy (LSCM) was used to evaluate the invasion and migration abilities of SACC-2 cells at different time point. Immunohistochemistry staining revealed that the expression of PCNA was significantly decreased (P <0.01) after salidroside treatment. In contrast, salidroside treatment led to increased caspase 3 and caspase 8 in SACC-2 cells. Cell migration depth and number of cells that penetrated Boyden chamber decreased also by salidroside. Salidroside potently inhibits the proliferation and then induces the apoptosis of SACC-2 cells. Migration and invasion of SACC-2 cells are also inhibited. Our data throw light on potential clinical application o f salidroside to the patients with SACC.