论文部分内容阅读
目的探讨PTEN表达与肝细胞癌发生、发展的关系,观察体外转染外源性PTEN对肝癌细胞的生长、细胞周期和超微结构的影响。方法检测PTEN在肝癌组织和肝癌细胞系中的表达缺失情况,以观察该基因与肝癌病理分级、转移和预后的关系。将含PTEN的反转录病毒载体及空载体转入人肝癌细胞系HHCC,观察转染前后细胞的生长、超微结构、细胞周期及裸鼠致瘤能力改变情况。结果肝癌中PTEN表达缺失率为30.16%(19/63),显著高于正常肝组织0(0/8)和肝硬化组织7.15%(1/14)。随着恶性程度的增高,PTEN表达缺失率随之增加。PTEN表达缺失与转移及预后显著相关。PTEN在人肝癌细胞系hHCC中也不表达。将抑癌基因PTEN转入后,hH-CC细胞生长明显受到抑制,细胞超微结构失去部分恶性表型特征,细胞周期由G1→S0期受抑制,并出现凋亡峰,体外成瘤能力下降。结论抑癌基因PTEN失表达多见于肝细胞肝癌进展期,并与肝细胞肝癌分化程度、是否转移以及临床预后相关;体外PTEN的转入可以明显地抑制HHCC生长、体外成瘤能力。
Objective To investigate the relationship between the expression of PTEN and the occurrence and development of hepatocellular carcinoma (HCC) and to observe the effects of transfection of exogenous PTEN on the growth, cell cycle and ultrastructure of HCC. Methods The expression of PTEN was detected in hepatocellular carcinoma and hepatocellular carcinoma cell lines to observe the relationship between the gene and the pathological grade, metastasis and prognosis of hepatocellular carcinoma. The PTEN-containing retroviral vector and empty vector were transfected into human hepatocellular carcinoma cell line HHCC. The growth, ultrastructure, cell cycle and tumorigenic ability of the transfected cells were observed before and after transfection. Results The loss of PTEN in HCC was 30.16% (19/63), which was significantly higher than that in normal liver tissue (0/8) and cirrhosis (7.15%, 1/14). As the degree of malignancy increases, the loss of PTEN expression increases. PTEN expression loss and metastasis and prognosis were significantly correlated. PTEN is also not expressed in the human hepatoma cell line hHCC. After transfection of tumor suppressor gene PTEN, the growth of hH-CC cells was significantly inhibited, and the ultrastructure of the cells lost some malignant phenotypes. The cell cycle was inhibited from G1 → S0, and the peak of apoptosis appeared. The in vitro tumorigenic ability decreased . Conclusions The loss of expression of tumor suppressor gene PTEN is more prevalent in hepatocellular carcinoma and is related to the degree of differentiation, metastasis and clinical prognosis of hepatocellular carcinoma. Transfection of PTEN in vitro can significantly inhibit the growth of HHCC and the ability of tumorigenesis in vitro.