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比较不同鸡蛋白蛋白（ Ｃ Ｅ Ａ）柱对 ３０ 份正常生育力精液进行精子优选的效果。分别以 ７５％单层 一步法（ Ｄ Ｃ）、７５％ 和１２５％ 双层一步法（ Ｓ Ｃ）及 ７５％ 预 选（ Ｆ Ｄ）再经 １０％ 、１５５％ 和 １７５％ 三层二步法（ Ｔ Ｃ） Ｃ Ｅ Ａ 柱进行优选。比较优选前后精子活动率、前向运动精子百分率和畸形率等常规指标及优选后各组精子回收率，并以盐酸喹啉染色比较优选前后精子 ｙ 小体阳性率。结果：优选后各组精子常规指标显著提高，以 Ｆ Ｄ 组精子回收率最高（３４７９％ ），高于上游法进行优选的回收率，与用人血清白蛋白进行优选的效果相当，可作为临床精子优选的有效手段。 Ｄ Ｃ、 Ｓ Ｃ 和 Ｔ Ｃ 各组回收率在 ２％ ～９％ 之间，显著低于 Ｆ Ｄ 组，但 ｙ 小体阳性 率均较优选前显著提 高， 在通过 Ｘ、 Ｙ 精子 分离以预防 Ｘ 连锁遗 传病上的可能用途有待进一步研究。
The effect of different egg albumin (C E A) columns on sperm optimization of 30 normal fertility semen was compared. The data were analyzed by 7 5% single layer one step method (D C), 75% and 125% double layer one step method (S C) and 75% % And 17.5% three-layer two-step (T C) C E A column. Pre-and post-sperm motility, percentage of forward motile sperm and abnormal rate of spermatozoa were compared before and after sperm motility, sperm recovery rate of each group was optimized. The positive rate of y-body before and after sperm motility was compared with quinoline hydrochloride. Results: The sperm routine indexes of each group were significantly improved, the recovery rate of sperm in F D group was the highest (3479%), the recovery rate higher than that of the upstream method was the same as that of human serum albumin, Clinical sperm optimization of effective means. The recovery rates of D C, S C and T C in each group were between 2% and 9%, which were significantly lower than those in F D group. However, the positive rate of y body was significantly higher than that before optimization. Possible uses of X-linked genetic diseases are for further study.