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目的了解广州市某医院2003年秋冬季儿童腹泻中诺瓦克样病毒(NLVs)感染的流行病学特点及病毒的基因型。方法收集2003年10月至2004年1月临床诊断为病毒性腹泻而就诊的患儿粪便标本,采用引物JV12Y/JV13I进行逆转录-聚合酶链反应检测NLVs,阳性标本的PCR产物经纯化、测序进行基因分析。结果收集的358份标本中检测出NLVs42份,阳性率11.73%。3岁以下的婴幼儿阳性40例,占95.24%,其中6月龄~1岁组与1~2岁组阳性率最高,分别占38.10%、35.71%。年龄最小者只有25天龄。4个月中,11月阳性率最高为17.27%,其次是2004年1月、2003年10、12月,阳性率分别为15.79%、8.11%、11.36%。抽取11份阳性标本进行测序分析,结果5份为GⅡ-3群,5份为GⅡ-4群,1份为GⅡ型,但尚不确定所属群。随机抽取20份轮状病毒阳性标本检测NLVs,未发现合并感染。结论广州市某医院儿童腹泻的散发与NLVs感染有关,并且存在着不同的基因型,流行优势株为GⅡ-3群与GⅡ-4群的病毒株。
Objective To understand the epidemiological characteristics and virus genotype of Norovirus-like virus (NLVs) infection in children’s diarrhea in a hospital in Guangzhou in the autumn of 2003. Methods Stool samples of children with clinical diagnosis of viral diarrhea were collected from October 2003 to January 2004. The positive samples were purified by PCR using primers JV12Y / JV13I and reverse transcriptase-polymerase chain reaction (PCR) Gene analysis. Results 358 samples collected detected NLVs 42, the positive rate of 11.73%. The positive rate of infants and children under 3 years old was 40 cases, accounting for 95.24%. Among them, the positive rates of 6 months old to 1 year old group and 1 to 2 years old group were the highest, accounting for 38.10% and 35.71% respectively. The youngest is only 25 days old. In 4 months, the highest positive rate in November was 17.27%, followed by January 2004 and October and December 2003 respectively. The positive rates were 15.79%, 8.11% and 11.36% respectively. Eleven positive samples were extracted and sequenced. Results showed that 5 were GⅡ-3, 5 were GⅡ-4, and 1 was GⅡ, but they were not identified. Twenty rotavirus positive samples were randomly selected to detect NLVs. No co-infection was found. Conclusion The distribution of children’s diarrhea in a hospital in Guangzhou is related to the infection of NLVs, and there are different genotypes. The predominant strains are the strains of GⅡ-3 and GⅡ-4.