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利用植物细胞培养离体筛选生物技术,以稻瘟病菌粗毒素提取液作为外源胁迫因素,对水稻种子体细胞和花粉性细胞无性系,进行愈伤组织细胞培养抗性筛选或花药培养抗性筛选。获得JR19等抗稻瘟病突变体,其抗性反应及酯酶同工酶谱与原品种具有明显差异。R_2代部份抗病突变体在R_3代仍然保持对苗稻瘟的抗性。抗病突变体大多伴随着株高、熟期、育性或粒重等农艺性状的变异。多数突变体在R_2代性状基本整齐一致,R_3代则保持相对稳定不变。讨论了抗性突变体离体筛选效果及其在抗病育种上的应用。初步提出水稻抗性突变体离体筛选程序。
In vitro screening of biotechnology using plant cell culture, crude extracts of Magnaporthe grisea as exogenous stress factors, the somatic cells of rice seeds and pollen cell clones, callus cell culture resistance screening or anther culture resistance filter. Accession to JR19 and other blast-resistant mutants, the resistance reaction and esterase isozyme patterns and the original varieties have significant differences. Some disease-resistant mutants of R_2 remained resistant to rice blast during the R_3 generation. Disease-resistant mutants are mostly associated with plant height, maturity, fertility or grain weight and other agronomic traits variation. Most of the mutants showed basically the same traits in the R_2 generations, while the R_3 generations remained relatively stable. In vitro screening of resistant mutants and their application in disease-resistant breeding were discussed. The preliminary screening of rice resistance mutant in vitro screening program.