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对所采集筛选的漂浮绿藻进行了定性和定量培养实验,并对筛选的绿潮漂浮种进行了ITS序列分析。根据实验结果,并未见有孢子或配子产生;对漂浮种的定期形态学显微观察也未见有成熟孢子囊或配子囊形成,但藻体的生物量一直处于增加状态。在实验条件下,生物量在30 d内增加约31倍。ITS序列系统进化树显示,QF1和QF2均被分在LPP复合体中,且亲缘关系较近,应属同一种类,但在外形上相差甚大。藻体的生长增殖一般情况下被并入自然生长,属于藻体自身长大。但实验藻体如其他绿潮优势种一样,均具有大量的分枝。分枝在从母体断裂或脱落后,成为新的母体进而进行增殖。这应当作为自体营养繁殖而成为绿潮漂浮种的另一种增殖方式,也是漂浮绿藻在适宜条件下生物量急剧增加的重要原因。
The qualitative and quantitative culture experiments of the collected floating green algae were carried out, and the ITS sequence analysis of the selected green tide floating species was carried out. According to the experimental results, no spores or gametes were found. No regular microscopic observation of the floating species showed the formation of mature sporangia or gamete, but the biomass of the algae was always increasing. Under the experimental conditions, the biomass increased about 31 times within 30 days. Phylogenetic tree of ITS sequence showed that both QF1 and QF2 were subdivided into LPP complex, and their genetic relationship was close and should belong to the same species, but the difference in appearance was very large. Growth and proliferation of algae are generally incorporated into the natural growth, belonging to the algae itself grow up. However, the experimental algae, like other dominant species of green tide, have a large number of branches. After the branches from the mother rupture or off, become a new mother and then proliferate. This should be another kind of propagation mode of green-tide floating species as autotrophic reproduction and also an important reason for the rapid increase of biomass of floating green algae under suitable conditions.