论文部分内容阅读
目的应用生物信息学软件预测结核分枝杆菌TrpD基因编码蛋白的结构和功能。方法应用Protparam程序预测TrpD蛋白的理化性质,利用SignaIP 4.1和MotifScan分析其信号肽和磷酸化位点,应用ClustalX、MEGA5.1软件分析TrpD蛋白的同源性等。结果 TrpD蛋白共370个氨基酸,分子式为C1652H2651N493O500S10,理论等电点为5.99,脂溶性系数93,不稳定系数28.86,预测该蛋白为稳定蛋白。TrpD蛋白无信号肽,其二级结构中β-转角占6.76%,无规则卷曲占32.7%。氨基酸序列中有5个潜在的B细胞抗原表位,13个Th细胞表位,22个磷酸化位点,与麻风杆菌和耻垢分枝杆菌的同源性分别为80.1%和76.6%。结论初步预测结核分枝杆菌TrpD为稳定蛋白,含有T、B细胞抗原表位,为抗结核新药的潜在作用靶标。
Objective To predict the structure and function of TrpD gene encoded by Mycobacterium tuberculosis using bioinformatics software. Methods The physical and chemical properties of TrpD protein were predicted by Protparam program. The signal peptide and phosphorylation sites of TrpD protein were analyzed by using SignaIP 4.1 and MotifScan. The homology of TrpD protein was analyzed by ClustalX and MEGA5.1 software. Results A total of 370 amino acids of TrpD protein, molecular formula C1652H2651N493O500S10, the theoretical isoelectric point of 5.99, fat solubility coefficient of 93, the coefficient of instability 28.86, the protein is predicted to be stable protein. TrpD protein no signal peptide, the secondary structure of β-turn accounted for 6.76%, irregular curl accounted for 32.7%. There are 5 potential B cell epitopes, 13 Th cell epitopes and 22 phosphorylation sites in the amino acid sequence, with 80.1% and 76.6% homology with M. leprae and M. smegmatis respectively. Conclusion The preliminary prediction of Mycobacterium tuberculosis TrpD as a stable protein, containing T, B cell epitopes, is a potential target for anti-TB drugs.