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查尔酮合成酶(chalcone synthase,CHS)是植物抗病抗逆过程中的一个关键酶。根据小麦抑制消减杂交中分离得到的小麦查尔酮合成酶基因片段,结合TAIL-PCR技术从小麦(Triticum aestivum L.)叶片中克隆得到查尔酮合成酶基因,命名为Ta CHS,其序列全长为2 543 bp,包含1 264 bp的启动子区、1 185 bp编码区和一个94 bp的内含子。分析显示该基因编码的氨基酸具有CHS家族的所有保守功能位点。同源性分析表明,Ta CHS与已报道的其他禾本科植物CHS基因编码的氨基酸序列同源性高达88%以上。启动子序列分析显示Ta CHS启动子区域具有光反应元件、植物激素响应元件、真菌诱导元件、M YB结合位点、TATA-Box和CAAT-Box等多种顺式作用元件。Ta CHS基因在全蚀菌侵染小麦后的表达开始上调,侵染后4 d达到最大值,之后开始下调。以上结果表明Ta CHS基因可能与小麦防御全蚀菌侵染有关。
Chalcone synthase (CHS) is a key enzyme in plant resistance to stress. The chalcone synthase gene was cloned from the leaves of wheat (Triticum aestivum L.) by TAIL-PCR and named Ta CHS based on the wheat chalcone synthase gene fragment isolated from the suppression subtractive hybridization It is 2 543 bp in length and contains 1 264 bp of promoter region, 1 185 bp of coding region and a 94 bp intron. Analysis showed that the gene encoded amino acids have all the conserved functional sites of the CHS family. Homology analysis showed that the homology between Ta CHS and the reported CHS gene of other gramineous plants was over 88%. Promoter sequence analysis showed that the Ta CHS promoter region has a variety of cis-acting elements such as photoreactive elements, plant hormone responsive elements, fungal inducing elements, M YB binding sites, TATA-Box and CAAT-Box. The expression of Ta CHS gene began to increase after total erosive inoculation of wheat and reached the maximum on the 4th day after infection and then began to decrease. The above results indicated that Ta CHS gene may be involved in the defense of wheat against eutrophication.