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利用ｆｕｒａ－２／ＡＭ荧光方法和放射免疫方法分别研究了脉冲电场对１１天和１４天胚龄的悬浮鸡胚脑细胞内Ｃａ２＋和环腺苷酸（ｃＡＭＰ）含量的影响，并探讨了在脉冲电场作用下这两种信号物质变化的相关性。实验结果表明：当电激液中［Ｃａ２＋］为１ｍｍｏｌ／Ｌ时，在宽度为１００微秒．强度为０．５，１．０，２．０ｋＶ／ｃｍ的脉冲电场作用下，１１天胚龄的鸡胚脑细胞内钙离子浓度（以［Ｃａ２＋］ｉ表示）与对照相比显著上升，其升高值随着脉冲强度的增加而增加，而细胞内ｃＡＭＰ含量与对照相比显著降低；用ＥＧＴＡ络合电激液中游离Ｃａ２＋以后，虽然细胞内［Ｃａ２＋］ｉ显著上升，细胞内ｃＡＭＰ含量没有显著变化。１４天胚龄的鸡胚脑细胞内［Ｃａ２＋］ｉ在脉冲电场作用下显著上升，而细胞内ｃＡＭＰ含量没有显著变化。脉冲电场对１１天和１４天胚龄的鸡胚脑细胞膜和细胞内Ｃａ２＋库Ｃａ２＋转移系统的开放均有显著作用，此作用具有对脉冲强度的依赖性。为了解物理信号跨细胞膜传导的方式提供了初步的实验根据
The effect of pulsed electric field on intracellular Ca2 + and cAMP content in suspended chicken embryo cells at eleven and fourteenth day embryonic days was studied by fura-2 / AM fluorescence and radioimmunoassay. The effects of pulsed electric field Correlation between the changes of these two signal substances under the electric field. The experimental results show that when the [Ca2 +] is 1 mmol / L, the width is 100 microseconds. The intracellular Ca2 + concentration (expressed as [Ca2 +] i) in 11-day-old chicken embryos increased significantly compared with the control under the pulse electric field of 0.5, 1.0 and 2.0 kV / cm. The increase of pulse intensity increased with the increase of pulse intensity, while the intracellular cAMP content decreased significantly compared with the control. After dissociated Ca2 + in EGTA, although intracellular [Ca2 +] i increased significantly, intracellular cAMP content No significant change. The [Ca2 +] i in chick embryo brain cells in 14-day-old embryos significantly increased under the action of pulsed electric field, while the intracellular cAMP content did not change significantly. The pulsed electric field had a significant effect on the opening of the chick embryo cell membrane and the intracellular Ca2 + pool Ca2 + transfer system at 11 and 14 days of embryonic age, and the effect was dependent on the pulse intensity. A preliminary experimental evidence is provided to understand the manner in which the physical signal travels across the cell membrane