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为了探讨人胎胰腺ＩＡＰＰ－与Ｉｎｓ－ＩＲ细胞的个体发生及其分布与共存，应用免疫组织化学ＰＡＰ法，对２２例１１～３０周人胎胰内ＩＡＰＰ－和Ｉｎｓ－ＩＲ细胞进行定位研究。结果显示，１１周人胎儿胰内已可见到ＩＡＰＰ－ＩＲ细胞单个散在，Ｉｎｓ－ＩＲ细胞较多，未见ＩＡＰＰ与Ｉｎｓ共存的细胞。１６周以后，ＩＡＰＰ－ＩＲ细胞逐渐增多，或散在分布，或三五成群。与邻片比较观察，可见到ＩＡＰＰ与Ｉｎｓ定位的细胞相应，但ＩＡＰＰ－ＩＲ细胞数量仍较Ｉｎｓ－ＩＲ细胞少，且免疫染色较弱。至３０周，在邻片同一胰岛内可见较多ＩＡＰＰ和Ｉｎｓ定位相同的细胞。研究结果提示，在胎儿期胰岛细胞已开始合成ＩＡＰＰ，并与Ｉｎｓ共存于Ｂ细胞，但ＩＡＰＰ与Ｉｎｓ两者在表达时序上有差异。本文还对ＩＡＰＰ与Ｉｎｓ在胎儿胰腺内共存的意义进行了讨论。
To investigate the occurrence, distribution and coexistence of IAPP- and Ins-IR cells in human fetal pancreas, immunohistochemical PAP method was used to study the localization of IAPP- and Ins-IR cells in 22 human embryonic pancreases of 11-30 weeks. The results showed that IAPP-IR cells were scattered in 11-week human fetal pancreas, there were more Ins-IR cells and no co-existing cells with IAPP and Ins. After 16 weeks, IAPP-IR cells gradually increased, or scattered or in small groups. Compared with neighboring cells, we can see that IAPP and Ins localized cells corresponded, but the number of IAPP-IR cells was still less than that of Ins-IR cells, and immunostaining was weak. By 30 weeks, more cells with the same orientation of IAPP and Ins could be seen in the same islet of the neighboring cells. The results suggest that in the fetal islet cells have begun to synthesize IAPP and coexist with Ins in B cells, but IAPP and Ins both in the timing of the expression differences. This article also discusses the significance of the coexistence of IAPP and Ins in the fetal pancreas.