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利用苎麻下胚轴高频再生体系,将携带人工合成的CryIA杀虫基因和CpTI基因的高效双价杀虫基因的植物表达载体pGBI4ABC转化到苎麻主栽品种中苎1号中。经根癌农杆菌侵染和共培养后,用50mgL-1卡那霉素+300mgL-1头孢霉素筛选共获得32株抗性植株;PCR检测显示26株为阳性,占80%。Southern杂交结果证实,外源基因已经整合到苎麻的基因组中。这项研究为最终创造兼抗鳞翅目及鞘翅目等害虫的苎麻种质材料奠定了基础。
The ramie hypocotyls high-frequency regeneration system was used to transform the plant expression vector pGBI4ABC, a highly efficient bivalent insecticidal gene carrying the synthetic CryIA insecticidal gene and the CpTI gene into Ramie No.1, a ramie host cultivar. After Agrobacterium tumefaciens infection and co-culture, 32 resistant plants were screened with 50mgL-1 kanamycin + 300mgL-1 cephalosporin. PCR test showed that 26 strains were positive, accounting for 80%. Southern hybridization results confirmed that foreign genes have been integrated into the ramie genome. This research laid the foundation for the ultimate creation of ramie germplasm material that is resistant to pests such as Lepidoptera and Coleoptera.