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为探索恶性疟原虫对新抗疟药苯芴醇的敏感性现场体外测定方法，提供敏感性数据，１９９７年８～１０月，应用Ｒｉｅｃｋｍａｎｎ体外微量测定法原理制作苯芴醇测定板，并在云南南部恶性疟流行现场测定了恶性疟原虫对该药的敏感性，重复２次测定的成功率分别为７３．８％（３１／４２）和７３．２％（３０／４１）；ＩＤ５０依次为１５０ｎｍｏｌ／Ｌ及１３０ｎｍｏｌ／Ｌ；ＩＤ９５为７４０ｎｍｏｌ／Ｌ及７６０ｎｍｏｌ／Ｌ；平均抑制裂殖体形成的浓度为４２２ｎｍｏｌ／Ｌ及４２３ｎｍｏｌ／Ｌ。测定成功率与氯喹板相同，疟原虫在各浓度药井中的抑制率亦同氯喹一样，随药物浓度的增高而依次上升，而裂殖体形成的例数则依次减少。提示本文研制的苯芴醇测定板药性稳定，重复性好，测定结果可靠，可用于恶性疟原虫对该药敏感性体外测定。
In order to explore the sensitivity of P. falciparum to lumeflufenol against new antimalarial drug in vitro and in vitro methods to provide sensitive data, from August to October 1997, the application of Rieckmann in vitro microassay principle of making lumefantrine assay plate, and in Yunnan The susceptibility of Plasmodium falciparum to the drug was measured in the southern part of P. falciparum. The success rates of two tests were 73.8% (31/42) and 73.2% (30/41), respectively. The ID50 was 150 nmol / L and 130nmol / L; ID95 was 740nmol / L and 760nmol / L; the average inhibition of schizonts formation was 422nmol / L and 423nmol / L. The success rate of determination was the same as that of chloroquine board. Plasmodium had the same inhibitory rate as that of chloroquine in various drug concentration wells, and then increased with the increase of drug concentration, while the number of schizonts formed decreased in turn. It is suggested that the meflumetol assay plate developed in this paper is stable, reproducible and reliable. The assay can be used for the in vitro determination of susceptibility of P. falciparum to this drug.