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系统性硬皮病(SSc)中转化生长因子β(TGFβ)和真皮、内脏病理性纤维变性有关。SSc初期损害中炎性细胞增多,并释放出TGFβ。但是,对硬皮病患者活检标本和血液标本测定TGFβ及相关信息蛋白,得出的结果并不一致。本次研究中对弥漫性、局限性SSc和硬斑病(morphea型)的临床上未受累皮肤和受累皮肤进行连续取材活检。把受累和未受累皮肤的细胞外TGFβ_1免疫细胞化学染色模式,与表面有HLA-DR和XⅢa因子标记的炎症细胞分布情况比较。并把染色的结果与前胶原阳性的成纤维细胞比较。另外,还测定这些患者血中TGFβ的水平。
Systemic scleroderma (SSc) transforming growth factor beta (TGFβ) and dermis, visceral pathological fibrosis related. Inflammatory cells increase in the initial lesion of SSc and release TGFβ. However, the results obtained from biopsy specimens and blood specimens from patients with scleroderma for the determination of TGFβ and related information proteins are not consistent. In this study, biopsies were taken from clinically unaffected skin and affected skin of diffuse, localized SSc and hard spot disease (morphea type). The pattern of immunocytochemical staining of extracellular TGFβ 1 in affected and uninvolved skin was compared with the distribution of inflammatory cells labeled with HLA-DR and XⅢa on the surface. The staining results were compared with those of procollagen-positive fibroblasts. In addition, the levels of TGFβ in the blood of these patients were also determined.