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目的探讨高压氧（ＨＢＯ）对大鼠急性局灶性脑缺血再灌注（ＩＲ）损伤的影响。方法应用血管内细丝栓堵大脑中动脉（ＭＣＡ）的局灶性脑缺血大鼠模型，利用组织病理学方法，观察了ＭＣＡ缺血１小时，再灌注４，１１，２３，７１小时脑梗塞区范围、神经元损伤程度及中性白细胞浸润的变化，并在常压纯氧治疗组和ＨＢＯ组分别应用常压纯氧（９９．５％）和０．２５ＭＰａ（ＡＴＡ）ＨＢＯ于再灌注期间各分别治疗１，２，３，５次后比较观察各组以上各时间梗塞区范围、神经元损伤程度及中性白细胞浸润的变化。结果常压纯氧组和缺血再灌注（ＩＲ）组相比梗塞区范围、神经元损伤程度及中性白细胞浸润无明显差异；而ＨＢＯ组和ＩＲ组相比梗塞灶面积和神经元损伤数目在再灌注７１小时（治疗５次后）分别减少了８．４３％（视交叉前平面）、１１．２０％（视交叉平面）、８．６６％（视交叉后平面）和每５个高倍（×４００）视野１１．３６个（视前区）、８．９４个（纹状体内侧区）、１４．２５个（皮层区），两组间存在显著性差异（Ｐ＜０．０５）。视交叉平面的视前区、纹状体与皮层的中性白细胞在ＭＣＡ缺血后５小时出现，分别为每５个高倍（×１００）视野３．８４、４．６１与２．５６个，在１２小时轻度增?
Objective To investigate the effect of hyperbaric oxygen (HBO) on acute focal cerebral ischemia-reperfusion (IR) injury in rats. Methods The model of focal cerebral ischemia was established by plugging the middle cerebral artery (MCA) with intravascular filament plug. The histopathological method was used to observe the MCA ischemia for 1 hour and the reperfusion for 4, 11, 23 and 71 hours Infarct area, neuron damage and neutrophil infiltration. The rats were treated with normobaric oxygen (99.5%) and 0.25 MPa (ATA) HBO in reperfusion During each period 1,2,3,5 times after treatment were observed in each group above the infarct area at each time range, the degree of neuronal damage and neutrophil infiltration changes. Results There were no significant differences in infarct size, neuronal damage and neutrophil infiltration between normobaric pure oxygen group and ischemia-reperfusion group. Compared with IR group, infarct size and neuronal damage Decreased by 8.43% (anterior occipital plane), 11.20% (occipital plane), 8.66% (posterior occipital plane), and every 5 high occipitations at 71 hours of reperfusion (after 5 treatments) There were significant differences (P <0.05) between the two groups (P <0.05), visual field of 11.36 (preoptic area), 8.94 (medial striatum) and 14.25 (cortical area) . The preoptic area of the optic chiasm, striatum and cortical neutrophils appeared 5 hours after MCA ischemia, with 3.84, 4.61 and 2.56 visual fields per 5 high magnification (× 100), respectively, Increase slightly in 12 hours?