论文部分内容阅读
目的:研究两种体外温育的晚期糖基化终产物(AGE)是否可以诱导星型胶质细胞分泌白介素-1β和肿瘤坏死因子α,并导致氧应激增加、一氧化氮释放.方法:RT-PCR技术检测两种细胞因子水平及AGE受体(RAGE)存在与否;DTNB反应测量还原型谷胱甘肽的水平;利用Griess试剂测量一氧化氮含量.结果:AGE 1 g/L(尤其是半乳精温育产物)作用72小时后使星型胶质细胞培养上清和细胞裂解物的细胞因子含量显著升高.且呈剂量依赖性.半定量RT-PCR证明两种细胞因子的变化是由于其转录水平增加所致.AGE还可导致星型胶质细胞内还原性谷胱甘肽减少,一氧化氮释放.RAGE存在于此类星型胶质细胞中.结论:AGE可诱导星型胶质细胞分泌炎性因于白介素-1β和肿瘤坏死因子α,并升高氧应激水平,这至少部分解释了AGE在神经变性性疾病如阿尔采默病和帕金森氏病以及脑衰老中的负性作用.
OBJECTIVE: To investigate whether two advanced glycation end products (AGEs) incubated in vitro can induce interleukin-1β and tumor necrosis factor-α secreted by astrocytes and lead to increased oxygen stress and release of nitric oxide.Methods: RT-PCR was used to detect the levels of two cytokines and the presence or absence of AGE receptor (RAGE); DTNB assay was used to measure the level of reduced glutathione; Nitric oxide was measured by Griess reagent.Results: AGE 1 g / L Especially the product of galactophore incubation), significantly increased the cytokines contents of astrocyte culture supernatant and cell lysate in a dose-dependent manner.A semi-quantitative RT-PCR proved that the cytokines of both cytokines The change is due to the increase of transcription level.AGE can also lead to reduction of glutathione, release of nitric oxide in astrocytes.RAGE is present in such astrocytes.Conclusion: AGE can induce Astrocytes secrete inflammatory mediators of interleukin-1β and tumor necrosis factor α and increase the level of oxygen stress, which at least partly explains the role of AGEs in neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease as well as brain The negative effect of aging.