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目的:探讨慢性丙型肝炎患者HCV感染与脂类代谢的关系及特点。方法:采用免疫沉淀法,以羊抗人载脂蛋白B(Apo-B)抗血清沉淀HCV抗体阳性者血清Apo-B,再以PCR技术进行扩增检测Apo-B中HCV-RNA,并设多组阴性对照。结果:实验组15例HCV抗体阳性者血清Apo-B中,14例有HCV-RNA表达,且载量较高,而对照组26例血清及其Apo-B中却没有HCV-RNA表达。结论:HCV抗体阳性者血清Apo-B中检出HCV-RNA,进一步证实了HCV感染与脂质密切相关。
Objective: To investigate the relationship between HCV infection and lipid metabolism in patients with chronic hepatitis C Methods: Apo-B was precipitated by anti-human apolipoprotein B (Apo-B) antiserum by immunoprecipitation and then detected by PCR to detect HCV-RNA in Apo-B Multiple negative control. Results: Of the 15 HCV-positive patients in the experimental group, HCV-RNA was expressed in 14 of the serum Apo-B patients, and HCV-RNA was not expressed in 26 serum samples and Apo-B in the control group. Conclusion: HCV-RNA was detected in serum of Apo-B patients with positive HCV antibody, further confirming that HCV infection is closely related to lipid.