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AIM:To investigate the expression of immunoglobulin geneSNC73 in malignant tumors and non-cancerous normaltissues.METHODS:Expression level of SNC73 in tumors and non-cancerous tissues from the same patient was determinedby reverse transcription polymerase chain reaction andenzyme-linked immunosorbent assay (RI-PCR-ELISA) in 90cases of malignant tumors,including colorectal cancer,gastriccancer,breast cancer,lung cancer and liver cancer.Analysison the correlation of SNC73 expression with sex,age,site,grade of differentiation,depth of invasion,and metastasesin colorectal cancer patients was made.RESULTS:Expression level of SNC73 in non-cancerouscolorectal mucosa and colorectal cancerous tissues was1.234±0.842 and 0.737±0.731,respectively (P<0.01),withthe mean ratio of 7.134±14.092 (range,0.36-59.54).Expression of SNC73 showed no significant difference amonggastric cancer,breast cancer,lung cancer and liver cancerwhen compared with non-cancerous tissues (P>0.05).Nocorrelation was found between SNC73 expression level andvarious clinicopathological factors,including sex,age,site,grade of differentiation,depth of invasion and metastasesof CRC patients.CONCLUSION:Down-regulation of SNC73 expression maybe a relatively specific phenomenon in colorectal cancer.SNC73 is a potential genetic marker for the carcinongenesisof colorectal cancer.The relationship of SNC73 expressionand carcinogenesis of colorectal cancer merits further study.
AIM: To investigate the expression of immunoglobulin geneSNC73 in malignant tumors and non-cancerous normal tissue. METHODS: Expression level of SNC73 in tumors and non-cancerous tissues from the same patient was determined by reverse transcription polymerase chain reaction andenzyme-linked immunosorbent assay (RI- PCR-ELISA) in 90cases of malignant tumors, including colorectal cancer, gastric cancer, breast cancer, lung cancer and liver cancer. Analysis of the correlation of SNC73 expression with sex, age, site, grade of differentiation, depth of invasion, and metastasesin colorectal cancer patients were made .RESULTS: Expression level of SNC73 in non-cancerous colorectal mucosa and colorectal cancerous tissues was 1.234 ± 0.842 and 0.737 ± 0.731, respectively (P <0.01), with the mean ratio of 7.134 ± 14.092 (range, 0.36-59.54) .Expression of SNC73 showed no significant difference among gastric cancer, breast cancer, lung cancer and liver cancer compared with non-cancerous tissues (P> 0.05) .Nocorrelation was found between SNC73 expression level andvarious clinicopathological factors, including sex, age, site, grade of differentiation, depth of invasion and metastases of CRC patients. CONCLUSION: Down-regulation of SNC73 expression maybe a relatively specific phenomenon in colorectal cancer. for the carcinongenesis of colorectal cancer. the relationship of SNC73 expression and carcinogenesis of colorectal cancer merits further study.