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目的研究人肝癌细胞株受照射后发生凋亡的时间、剂量关系。方法用ＴＵＮＥＬ及流式细胞仪方法观察ＱＧＹ－７７０３人肝癌细胞株受照射后凋亡情况。结果ＱＧＹ－７７０３细胞株自发凋亡率为４．２２％，受照射后凋亡发生随剂量增加和温育时间延长而增加。当剂量达８Ｇｙ或温育时间达２４小时趋于稳定。结论与淋巴造血系统来源的瘤株相比，人肝癌细胞株自发凋亡较低，受照射后增加较少，提示肝癌中凋亡易感细胞比例较低
Objective To study the time and dose relationship of apoptosis in human hepatoma cell lines after irradiation. Methods The apoptosis of QGY-7703 human hepatocellular carcinoma cell line after irradiation was observed by TUNEL and flow cytometry. Results The spontaneous apoptotic rate of QGY-7703 cells was 4.22%. After irradiation, the apoptosis increased with increasing dose and incubation time. When the dose reaches 8Gy or the incubation time reaches 24 hours, it stabilizes. Conclusion Compared with the lymphoid hematopoietic system-derived tumor strains, the spontaneous apoptosis of human hepatoma cell lines was lower and less after irradiation, suggesting a lower proportion of apoptotic susceptible cells in hepatic cancer cells.