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在pH=3.0的B-R缓冲介质中,人血清白蛋白(HSA)与四溴荧光黄(TBF)结合,形成TBF-HSA复合物并被石蜡微晶吸萃,以试剂空白参比,在555nm处产生一灵敏的反射散射吸收峰。HSA的浓度在0.0—1.2mg/L范围内与555nm处的反射散射吸收值呈良好的线性关系。方法对HSA的检出限为0.058mg/L,本方法灵敏,可用于人血清样品中蛋白质的测定。
Human serum albumin (HSA) binds to tetrabromofluorescein (TBF) in a BR buffering medium at pH = 3.0 to form a TBF-HSA complex and is absorbed by a paraffin microcrystal with a reagent blank reference at 555 nm A sensitive reflection scattering absorption peak results. HSA concentration in the range of 0.0-1.2mg / L and 555nm at the reflection scattering absorption value showed a good linear relationship. The detection limit of HSA was 0.058mg / L. The method is sensitive and can be used for the determination of protein in human serum samples.