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目的 :研究运动与心脏重塑过程中心肌胞浆游离Ca2 +([Ca2 +]c)动态变化的生物学机制。方法 :采用激光扫描共聚焦显微技术 (LSCM)对STDInCa2 +荧光试剂负载的运动肥大心肌活细胞 [Ca2 +]c 动态变化进行研究 ,采用放射免疫测定运动小鼠心肌局部IGF -Ⅰ和AngⅡ含量。结果 :运动肥大心肌 [Ca2 +]c 变化表现为基值稳态和峰值显著升高 ,达峰时间延长 (P <0 0 0 1)。心肌局部IGF -Ⅰ和AngⅡ显著升高 (P <0 0 0 1)。结论 :运动性心肌肥大与 [Ca2 +]c 变化、机械信号、IGF -Ⅰ和AngⅡ关系密切 ,机械信号、IGF -Ⅰ和AngⅡ可能是引起 [Ca2 +]c 显著升高 ,增强心肌收缩能力的胞外刺激因素。
OBJECTIVE: To study the biological mechanism of the dynamic change of free Ca2 + ([Ca2 +] c) in myocardial cytoplasm during exercise and cardiac remodeling. Methods: Dynamic changes of [Ca2 +] c in living hypertrophic myocardium loaded with STDInCa2 + fluorescent reagent were studied by laser scanning confocal microscopy (LSCM). The contents of IGF-Ⅰ and AngⅡ in myocardium were measured by radioimmunoassay . Results: The changes of [Ca2 +] c in hypertrophic myocardium showed the steady state and the peak value increased significantly and the peak time prolonged (P <0.01). Myocardial IGF-Ⅰ and AngⅡ were significantly increased (P <0.01). CONCLUSION: Exercise induced cardiac hypertrophy is closely related to changes of [Ca2 +] c, mechanical signals, IGF-I and AngII, and mechanical signals, IGF-I and AngII may induce a significant increase of [Ca2 +] c and enhance myocardial contractility Extracellular stimuli.