论文部分内容阅读
3.姐妹染色单体互换(sister chromatid exchange,SCE)SCE是细胞遗传学中极敏感的指标。近年来对SCE的影响因素有较多研究。血液抗凝剂以肝素为参照,发现EDTA可使SCE升高,EDTA和柠檬酸右旋糖使复制指数和分裂指数下降。人体全血或提纯的淋巴细胞在-100℃下冷冻贮藏1～6个月,对SCE的本底值和诱发频率无明显影响。此结果为远距离传递血样提供了依据。在职业人群监测中必要时可使用血液的冷冻贮藏技术。4种
3. Sister chromatid exchange (SIE) SCE is a very sensitive cytogenetic marker. In recent years, the impact of SCE factors have more research. Blood anticoagulation with heparin as a reference, found that EDTA can make SCE increased, EDTA and dextrose citrate so that the replication index and mitotic index decreased. Human whole blood or purified lymphocytes stored frozen at -100 ° C for 1 to 6 months had no significant effect on the SCE background and the frequency of induction. This result provides the basis for transmitting blood samples over long distances. In the monitoring of the occupational population may use the blood of frozen storage technology if necessary. 4 kinds