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为探讨人白细胞介素4（hIL－4）cDNA修饰对人髓系白血病株HL－60细胞体外生物学特性的影响，构建了hIL－4重组逆转录病毒载体。用脂质体转染法将其导入包装细胞获高效价病毒上清，用其感染HL－60细胞株并筛得hIL－4高表达克隆5株，用~3H－TdR掺入法检测其增殖反应及其诱导的正常人PBMC增殖反应。结果显示，IL－4高表达克隆体外增殖反应明显强于低表达克隆；丝裂霉素处理后，前者诱导的正常人PBMC增殖反应明显强于后者，此增强作用在IL－4作用48h后不被IL－4单抗阻断。提示IL－4基因修饰可能提高HL－60细胞的免疫原性。
HIL-4 recombinant retroviral vector was constructed to investigate the effect of human interleukin-4 (hIL-4) cDNA modification on the biological characteristics of human myeloid leukemia HL-60 cells in vitro. The recombinant plasmid was transformed into HL-60 cell line by lipofection method and then transfected into packaging cells. Five clones with high expression of hIL-4 were screened and the proliferation of HL-60 cells was detected by 3H-TdR incorporation assay Response and its induced normal human PBMC proliferative response. The results showed that the proliferation of IL-4-overexpressing clones was significantly stronger than that of the low-expressing clones. After mitomycin-treated, the proliferation of normal human PBMC induced by IL-4 was obviously stronger than that of the latter, Not blocked by IL-4 mAb. It is suggested that IL-4 gene modification may enhance the immunogenicity of HL-60 cells.