论文部分内容阅读
探索鬼臼酰肼哌啶腙氮氧自由基（ＧＰ－１）中自由基对其抗肿瘤作用及毒性的影响．方法：采用小鼠移植肿瘤及体外培养的肿瘤细胞系，检测ＧＰ－１及其自由基还原物ＧＰ－１－Ｈ的体内外抗肿瘤作用，毒性及对细胞周期，有丝分裂指数和小鼠免疫功能的影响．结果： ＧＰ－１ １／６， １／１２ ＬＤ５０给药１０ｄ，对小鼠Ｌｅｗｉｓ肺癌， Ｓ１８０， Ｐ３８８和 ＨｅＰＳ生长的抑制作用，在体外对ＳＧＣ－７９０１细胞增殖和Ｌ１２１０细胞ＤＮＡ合成的抑制作用均比ＧＰ－１－Ｈ强．其对Ｌ１２１０细胞作用环节在细胞周期的Ｍ期之后．ＧＰ－１－Ｈ对细胞周期的影响与ＧＰ－１相似，但作用较弱．结论：ＧＰ－１中的自由基在增强其抗肿瘤作用中起着重要作用。
To explore the effects of free radicals in podophylloperazide piperidine hydrazone nitroxide (GP-1) on its antitumor activity and toxicity. Methods: The anti-tumor effect and toxicity of GP-1 and its GP-1-H free radical reductant were assayed in vitro and in vivo by using mouse transplanted tumor and in vitro cultured tumor cell lines. The cell cycle, mitotic index and immune function Impact. Results: The inhibitory effects of GP-1 1/6 and 1/12 LD50 on the growth of Lewis lung carcinoma S180, P388 and HePS mice in vitro were inhibited by both GP-1 1/6 and 1/12 LD50 for the proliferation of SGC-7901 cells and DNA synthesis of L1210 cells More than GP-1-H. Its role in L1210 cells in the M phase of the cell cycle. The effect of GP-1-H on the cell cycle is similar to that of GP-1, but its effect is weaker. Conclusion: Free radicals in GP-1 play an important role in enhancing its anti-tumor effect.