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目的通过比较聚合酶链反应偶联印迹杂交和酶联放大技术(即三联放大技术)与荧光定量PCR(FQ-PCR)两种方法检测HBV DNA结果,探讨三联放大技术用于血液HBV筛查的优越性。方法医院肝病门诊患者血清195份,同时进行三联放大技术检测和FQ-PCR检测,比较检测结果。结果195份血清,经三联放大技术检出HBVDNA 125份,检出率64.10%;FQ-PCR检出HBV DNA 103份,检出率52.82%。两种方法的HBV DNA检出率差异有统计学意义(P<0.01)。结论与FQ-PCR比较,三联放大技术能显著提高HBV DNA的检出率,可用于血液HBV筛查。
Objective To compare HBV DNA results by polymerase chain reaction-linked blot hybridization and enzyme-linked amplification (triple-amplification) and fluorescence quantitative PCR (FQ-PCR), and to investigate the effect of triplex amplification on blood HBV screening Superiority. Methods A total of 195 serum samples were collected from patients with liver disease outpatient in our hospital. Triplex amplification and FQ-PCR were performed simultaneously to compare the results. Results 195 serum samples were detected by triplex amplification, 125 of them were detected by PCR, and the detection rate was 64.10%. FQ-PCR detected 103 copies of HBV DNA, the detection rate was 52.82%. The detection rates of HBV DNA between the two methods were statistically significant (P <0.01). Conclusion Compared with FQ-PCR, triple amplification can significantly improve the detection rate of HBV DNA and can be used for blood HBV screening.