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目的:研究环丙沙星和致病菌铁结合蛋白之间的作用,探索致病菌铁结合蛋白(FBP)是否为环丙沙星的靶分子。方法:以紫外-可见分光光度法和核磁共振法,观察环丙沙星与从大肠杆菌表达出的淋病奈瑟双球菌的铁结合蛋白相互作用的结果。结果:在10mmol/L、pH7.40Tris-HCl缓冲溶液中,298K时,环丙沙星与Fe3+形成配合物而将Fe3+从holo-FBP中脱去,当环丙沙星过量50倍时,可移去holo-FBP中一半量的Fe3+。结论:环丙沙星能部分脱去holo-FBP中的Fe3+,从而可干扰细菌对Fe3+的获取,进而可影响细菌的生长和致病能力。
Objective: To study the interaction between ciprofloxacin and iron-binding protein of pathogenic bacteria and explore whether FBP is the target of ciprofloxacin. Methods: The interaction between ciprofloxacin and iron-binding protein of Neisseria gonorrhoeae expressed from Escherichia coli was observed by ultraviolet-visible spectrophotometry and nuclear magnetic resonance. Results: In the buffer solution of 10 mmol / L, pH7.40Tris-HCl, the conjugate of ciprofloxacin with Fe3 + and the removal of Fe3 + from holo-FBP at 298K, Remove half of the amount of Fe3 + in the holo-FBP. Conclusion: Ciprofloxacin can partially remove Fe3 + from holo-FBP, which can interfere with the bacteria’s access to Fe3 +, which may affect the growth and pathogenicity of bacteria.