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1 对象和方法1.1 随机抽取12家医疗单位,其中市级医院4家,乡镇医院8家。监测内容:空气(手术室、分娩室、供应室)中的细菌总数、乙型溶血性链球菌;物体表面(手术床、工作室)中细菌总数、大肠菌群、金黄色葡萄球菌;手(医生、护士)中的细菌总数、大肠菌群、金黄色葡萄球菌;压力蒸气灭菌效果监测(嗜热脂肪芽胞杆菌)。1.2 方法。1.2.1 空气检测:采用沉降法(平板直径为9cm),采样设点按东西南北中5个点(<30m~2对角线设3个点)各放一普通营养琼脂平板和血平板,暴露5min后,置37℃温箱,培养24h,观察结果。
1 objects and methods 1.1 randomly selected 12 medical units, including 4 municipal hospitals, township hospitals 8. Monitoring contents: total number of bacteria in air (operating room, delivery room, supply room), hemolytic streptococcus B; total number of bacteria, coliforms and Staphylococcus aureus in the surface of an object (operating table, studio) Doctors, nurses), coliform, Staphylococcus aureus; pressure steam sterilization effect monitoring (Bacillus stearothermophilus). 1.2 method. 1.2.1 Air Detection: The sedimentation method (plate diameter of 9cm), the sampling set point according to the five points in the east, west, north and south (3 points <30m ~ 2 diagonal) put a normal nutrient agar plate and blood plate, After exposure for 5min, set 37 ℃ incubator, cultured 24h, the observation results.