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目的:利用人类幽门螺杆菌(H.pylori)感染的小鼠模型研究过氧化氢酶和黏附素保守区二价疫苗治疗H.pylori感染的作用.方法:把已感染H.pylori的小白鼠分成7组,分别通过灌胃方法给予过氧化氢酶和黏附素保守区(各100μg)加霍乱毒素CT(2μg)、过氧化氢酶(100μg)加CT(2μg)、黏附素保守区(100μg)加CT(2μg),PBS,单纯过氧化氢酶(100μg)、单纯黏附素保守区(100μg)、单纯CT(2μg),1次/wk,共4次,治疗结束后4wk处死动物,取胃黏膜行半定量细菌培养检查H.pylori情况.结果:治疗实验各组H.pylori根除率分别为:过氧化氢酶和黏附素保守区加CT组69.2%(18/26)、过氧化氢酶加CT组30.8%(8/26)、黏附素保守区加CT组38.5%(10/26);生理盐水组、单纯过氧化氢酶、单纯黏附素保守区、单纯CT组根除率均为0%.未根除H.pylori的小鼠,疫苗治疗组H.pylori的定植密度明显低于其他4组(P<0.05).此外,二价疫苗组的H.pylori根除率及定植密度较单价疫苗组均有显著性差异(P<0.05).结论:由过氧化氢酶和黏附素保守区加免疫佐剂组成的二价口服疫苗较单价口服疫苗有更好的免疫治疗效果,多价疫苗是未来H.pylori疫苗的研制方向.
OBJECTIVE: To study the effect of catalase and adhesin bivalent vaccine on H.pylori infection in mice infected by H. pylori infection.Methods: The H.pylori-infected mice were divided into (100 μg), CT (2 μg), catalase (100 μg) and CT (2 μg), catalase (100 μg) Animals were sacrificed 4 weeks after treatment with CT (2μg), PBS, catalase (100μg), simple adhesin (100μg), simple CT (2μg) and once / H.pylori was detected by mucosal semi-quantitative bacterial culture.Results: The H.pylori eradication rate in each group was 69.2% (18/26) in catalase and adhesin-conserved areas, catalase 30.8% (8/26) in the CT group and 38.5% (10/26) in the adhesion zone of CT and CT group. The eradication rates in the normal saline group, catalase, simple adhesin and CT group were all 0 The H.pylori colonization density of the vaccine-treated group was significantly lower than that of the other 4 groups (P <0.05) .In addition, H.pylori eradication rate and colonization in the bivalent vaccine group (P <0.05) .Conclusion: The bivalent oral vaccine composed of catalase and adhesin conserved region plus adjuvant is better immunotherapy than monovalent vaccine, Multivalent vaccine is the future direction of H.pylori vaccine development.