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关节软骨（细胞）的同种异体移植是解决关节软骨损伤后修复问题的理想方法．其成功的先决条件要求细胞能够耐受低温冷冻保存并保持关节软骨细胞的生物学特性。为此，该实验在成功地建立了关节软骨细胞库的基础上，进行冻存后的关节软骨细胞培养，同时利用显微缩时录像技术，借助组织学、组织化学和分子生物学等方法．观察细胞的增殖、糖胶聚糖的合成以及Ⅱ型胶原的基因表达情况。结果表明，分离的关节软骨细胞经深低温冷冻保存后复苏．细胞在14d的培养中仍可保持关节软骨细胞所特有的形态以及对糖胺聚糖和Ⅱ型胶原的合成能力。
Allograft of articular cartilage (cell) is the ideal method to solve the repair problem of articular cartilage injury. Its prerequisite for success requires that cells be able to tolerate cryopreservation and maintain the biological properties of articular chondrocytes. To this end, based on the successful establishment of an articular chondrocyte reservoir, the experiment was conducted to culture the articular chondrocytes after cryopreservation, and at the same time using microscopic time-lapse recording technology with the aid of histological, histochemical and molecular biology methods. The proliferation of cells, the synthesis of glycosaminoglycan and the gene expression of type Ⅱ collagen were observed. The results showed that the isolated articular chondrocytes were resuscitated after cryopreservation. Cells in the 14d of culture can still maintain the unique shape of articular chondrocytes and synthesis of glycosaminoglycans and type Ⅱ collagen.