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制备不同胎龄水囊引产胎脾Ｍ，以ＬＰＳ（１０ｍｇ／Ｌ）为诱导剂，刺激４８ｈ，ＭＴＴ法检测不同胎龄胎脾Ｍ上清中ＩＬ－１、ＩＬ－６、ＴＮＦ的含量，并与外伤成人脾Ｍ的分泌功能进行比较，发现不同的单核细胞团子，其胚胎发育状况极不一致：胎脾ＭＩＬ－１的分泌在胚胎３２周达成人水平（Ｐ＞０．０５）并保持至出生；ＩＬ－６的分泌在胚胎２０周时已与成人无差别（Ｐ＞０．０５），至出生前，已显著超过成人水平（Ｐ＜０．０１）；而ＴＮＦ的分泌，在胚胎期一直处于较低水平（Ｐ＜０．０１）。同时观察到免疫介质（ＬＰＳ、ＩＦＮ－γ）对胎脾Ｍ分泌ＩＬ－６、ＴＮＦ有协同刺激作用。
The fetus spleen M was induced by different gestational age water sacs and stimulated by LPS (10mg / L) for 48h. The content of IL-1, IL-6 and TNF in the M supernatants were determined by MTT assay Compared with the secretory function of spleen M in trauma adults, it was found that the embryonic development status of different mononuclear cell masses was extremely inconsistent. The secretion of fetal spleen MIL-1 reached the adult level at 32 weeks (P> 0.05) (P <0.05). The secretion of IL-6 was significantly higher than that of the adult before birth (P <0.01), while the secretion of TNF in the embryo Has been at a low level (P <0.01). At the same time, immune mediators (LPS and IFN-γ) were observed to synergistically stimulate IL-6 and TNF secreted by fetal M secretions.