论文部分内容阅读
已有研究表明，肾上腺髓质素（ＡＭ）对血糖和胰岛激素的分泌有明显影响。为了解ＡＭ结构和功能的关系，我们合成了ＡＭ１－１３、ＡＭ１６－５２、ＡＭ２３－５２和ＡＭ４０－５２四个多肽片段，并观察在大鼠静脉滴注时其对血糖、胰岛素和胰升糖素浓度的影响。实验结果表明：ＡＭ１６－５２和ＡＭ２３－５２均可使血糖降低、胰岛素分泌增加，但这４个片段对胰升糖素浓度均无影响。ＡＭ１－１３无降糖作用，但滴注２小时后能使血胰岛素升高。说明完整的ＡＭ分子存在一个较长的多肽片段，它可促进胰岛素分泌，定位在ＡＭ２３－４０左右。而ＡＭ分子中能抑制胰升糖素分泌的有效片段较短，在分段合成时被分割而失去活性。但对ＡＭ１６－５２和ＡＭ２３－５２的促胰岛素分泌作用的机理尚需进一步研究。
Studies have shown that adrenomedullin (AM) has a significant effect on the secretion of glucose and insulin. To understand the relationship between AM structure and function, we synthesized AM1-13, AM16-52, AM23-52 and AM40-52 four peptide fragments, and observed in rats intravenous infusion of glucose, insulin and pancreatic sugar The effect of prime concentration. Experimental results show that both AM16-52 and AM23-52 can reduce blood glucose and insulin secretion, but these four fragments have no effect on glucagon concentration. AM1-13 no hypoglycemic effect, but after 2 hours of instillation can make blood insulin. This shows that there is a longer peptide fragment in the intact AM molecule, which can promote insulin secretion and is located in the vicinity of AM23-40. However, the active molecules that inhibit glucagon secretion in AM molecules are shorter and are segmented and lose their activity when synthesized in segments. However, the mechanism of insulin secretion of AM16-52 and AM23-52 needs further study.