论文部分内容阅读
目的 评价第三代 β -受体阻滞剂卡维地洛对家兔急性心肌梗塞 (AMI)后的左室重构的防治作用 ,以及对梗死区损伤心肌细胞的修复。方法 采用家兔制备心肌梗死模型 ,2 0只AMI术后成活的雄性家兔随机分为AMI对照组、卡维地洛 (CVD)组 ,另设假手术组。灌胃给药 4周后处死动物。用电子精确天平称量左室、右室的重量 ,用羟脯氨酸方法测定心肌组织的胶原的含量及采集标本进行病理分析。结果 梗死后左心室、右心室重量均比假手术组增加 ,差异显著。梗死区胶原含量显著增加 ,非梗死区胶原含量增加明显。经卡维地洛治疗后 ,左心室、右心室重量均下降 ,但未能恢复至正常水平。梗死区胶原含量亦略有下降 ,但差异不显著。非梗死区胶原含量下降明显 ,差异显著。病理分析结果显示卡维地洛组心肌细胞损伤程度减轻。结论 卡维地洛能有效防治家兔AMI后的左室重构及减轻心肌细胞损伤程度。
Objective To evaluate the preventive and therapeutic effects of carvedilol, a third generation β - blocker, on left ventricular remodeling after acute myocardial infarction (AMI) in rabbits and the repair of injured myocardial cells in infarct area. Methods Rabbit models of myocardial infarction were prepared. Twenty male rabbits alive after AMI were randomly divided into AMI control group, carvedilol (CVD) group and sham operation group. Animals were sacrificed 4 weeks after gavage. The weight of left ventricle and right ventricle were weighed by electronic balance, the content of collagen in myocardial tissue was measured by hydroxyproline method and the pathological changes were collected. Results Left ventricle and right ventricular weight after infarction were significantly higher than those in sham operation group. Collagen content in infarct area increased significantly, collagen content in non-infarct area increased significantly. After carvedilol treatment, left ventricular and right ventricular weight decreased, but failed to return to normal levels. Collagen content in infarction area also decreased slightly, but the difference was not significant. Non-infarct collagen decreased significantly, the difference was significant. Pathological analysis showed that the degree of myocardial cell injury in carvedilol group decreased. Conclusion Carvedilol can effectively prevent left ventricular remodeling and mitigate the damage of myocardial cells after AMI in rabbits.