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本文首次建立了一个简单而准确的高效液相色谱法指纹图谱方法,对草木樨药材不同收获时间和不同的应用部分,包括种子,茎和整个地上部分进行了系统评价.结果采用线性梯度洗脱,乙腈和0.1%磷酸水为流动相,柱温25℃,检测波长为210 nm.该高效液相色谱指纹图谱表明,不同药用部分有12个相同的共有峰和8个相同的共有指纹峰.说明高效液相色谱指纹图谱可用于草木樨属植物质量评价,同时本文研究方法和思路也可用于其他中药的质量和分析研究.“,”A simple and accurate high-performance liquid chromatography method (HPLC) was described to generate the me-tabolite fingerprints of Melilotus, suaveolens Ledeb of different harvesting times and different parts of the plant. A suitable chro-matographic system was established using a linear gradient elution with acetonitrile and 0.1% phosphoric acid (H3PO4) as the mobile phase and a detection wavelength of 210 nm. HPLC analysis identified 12 common peaks and 8 common fingerprint peaks among the different parts of the herb. We found that the constituents varied at different harvesting times and in different parts of the plant. The HPLC fingerprint method could be used to evaluate the quality of M. Suaveolens Ledeb and Melilotus genus herbs. The same strategy can also be applied for the development of fingerprints for the quality control of other herbal medicines.