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中药黄芪为常用补气药,市场上黄芪药材来源复杂,质量参差不齐.本研究针对56份不同种源、不同栽培基地、不同年限、不同采收季节、不同商业规格的黄芪药材,采用高效液相色谱法,测定了其中的有效成分(毛蕊异黄酮、毛蕊异黄酮苷、芒柄花素、芒柄花苷及黄芪甲苷)的含量,对其进行质量评价.实验结果表明不同来源的黄芪药材的异黄酮类化合物及黄芪甲苷的含量存在明显的差异,为黄芪药材的选用提供了一定的依据.“,”Radix Astragali (Huangqi) is one of the most valuable traditional Chinese medicinal herbs. It is used in the traditional Chinese medicine to reinforce 'qi', and it has immunostimulant, tonic, and antioxidant activities. There are many different sources of Huangqi in the market. In this study, the quality of Huangqi was evaluated by the measurement of four flavonoids, calycosin-7-O-β-D-glucopyranoside, ononin, calycosin and formononetin with HPLC-UV, as well as astragaloside Ⅳ with HPLC-ELSD. Samples included different plant species, different places of cultivation, different ages of plants, different seasons of collection and different commercial specifications. The results showed that the contents of isoflavonoids and astragaloside Ⅳ varied significantly in different sources. Our study provided useful information for the quality evaluation of Radix Astragali.