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松墨天牛(MonochamusalternatusHope)是松树的重要蛀干害虫,也是松材线虫(Bursaphelenchusxylophilus)的主要传播媒介。试验从松墨天牛文库中分离得到肌肉LIM蛋白基因的部分cDNA序列,长度为871 bp,命名为MaLIM(GenBank:KJ872589);与赤拟谷盗(Tribolium castaneum)和家蚕(Bombyxmori)氨基酸相似性分别为77%和75%。用RT-qPCR测定了11种杀虫剂对MaLIM相对表达量的影响,结果表明,杀虫剂处理后MaLIM基因的表达量均有所下降,仅为对照的0.03~0.53。
Monochamus alternatus Hope is an important borer pest of pine and is also the main transmission medium of Bursaphelenchus xylophilus. The partial cDNA sequence of muscle LIM protein gene was isolated from Monochamus alternatus library and its length was 871 bp, named MaLIM (GenBank: KJ872589). The amino acid similarity with Tribolium castaneum and Bombyx mori 77% and 75% respectively. The effects of 11 insecticides on the relative expression level of MaLIM were determined by RT-qPCR. The results showed that the expression level of MaLIM gene decreased after treatment with insecticide, only 0.03-0.53 of the control.