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目的 研究小剂量(100 nmol/L)H_2O_2对大鼠肝卵圆细胞株WB-F344细胞膜理化特性、脂质过氧化及基因组DNA的影响。方法利用标记膜脂及膜蛋白质的荧光标记物1，6-二苯基-1，3，5-己三烯(DPH)和N-(3芘)马来酰亚胺[N-(3P)M]标记不同剂量H_2O_2作用及经H_2O_2多次作用后发生转化的WB细胞，通过测定其荧光偏振度确定膜理化特性的改变；以硫代巴比妥酸(TBA)法测定其脂质过氧化产物丙二醛（MDA）含量的变化；以及利用溴化乙锭(EB)作荧光探针，通过DNA-EB结合物荧光强度的变化确定其基因组DNA的损伤程度。结果小剂量(100 nmol/L)H2O2的一次作用可使膜蛋白运动度增加，膜脂流动性增大，MDA的含量增高；H_2O_2的多次作用使上述变化更为显著。大剂量(1 mmol/L)、中剂量(100 μmol/L)的H_2O_2作用细胞后可直接损伤基因组DNA，小剂量H2O2的一次作用虽然不能直接损伤基因组DNA，但多次作用后却可使基因组DNA受损。结论H_2O_2可诱使WB细胞膜理化特性发生改变，脂质过氧化产生及造成基因组DNA损伤，此可能与H_2O_2的致、促癌作用有关。
Objective To study the effects of low dose (100 nmol/L) H 2 O 2 on the physicochemical properties, lipid peroxidation and genomic DNA of hepatic oval cell line WB-F344. Methods The fluorescent labels labeled membrane lipid and membrane protein 1,6-diphenyl-1,3,5-hexatriene (DPH) and N-(3H) maleimide [N-(3P)] M] marked different doses of H2O2 and transformed WB cells after repeated H_2O_2 exposures. The changes in physicochemical characteristics of the membrane were determined by measuring the degree of fluorescence polarization; lipid peroxidation was measured by thiobarbituric acid (TBA) method. The content of malondialdehyde (MDA) in the product was changed; and the degree of DNA damage was determined by the change of the fluorescence intensity of the DNA-EB conjugate using ethidium bromide (EB) as a fluorescent probe. Results The effect of low-dose (100 nmol/L) H2O2 could increase the mobility of membrane proteins, increase the fluidity of membrane lipids, and increase the content of MDA. The multiple effects of H2O2 made the above changes more significant. High-dose (1 mmol/L) and medium-dose (100 μmol/L) H 2 O 2 cells can directly damage genomic DNA. Although a single dose of H 2 O 2 can not directly damage genomic DNA, multiple actions can make the genome possible. DNA damage. Conclusion H 2 O 2 can induce changes in the physicochemical properties of WB cell membrane, lipid peroxidation and genomic DNA damage, which may be related to H 2 O 2 induced and cancer-promoting effects.