论文部分内容阅读
已报道妊娠血清(PS)可非特异性地抑制对有丝分裂原的应答性及混合淋巴细胞反应(MLR)。MLR 中 T 细胞激活需有白细胞介素1(IL-1)及同种异体抗原。这二种刺激同时诱导 T_H 细胞合成白细胞介素2(IL-2),并诱导 IL-2受体阳性细胞进入 S 期。也已证实 PS 可抑制 T_H 增殖及 MLR 中IL-2合成。本文旨在分析 PS 对 IL-1的“抑制作用”,是通过影响其合成抑或是通过抑制其特异性活性所致。作者自孕龄8周的初次或多次妊娠孕妇收集血清,以男性供者血清即正常人血清(NHS)作为正常对照。以正常供者的外周血单核细胞(PBMC)作单向 MLR。将人 PBMC 加脂多糖(LPS)刺激,分别加入 PS 和 NHS,37℃培养24小时。收
Gestational sera (PS) have been reported to nonspecifically inhibit responsiveness to mitogens and mixed lymphocyte reaction (MLR). T cell activation in MLR requires interleukin 1 (IL-1) and alloantigen. Both of these stimuli induce both T_H cells to synthesize interleukin 2 (IL-2) and induce IL-2 receptor positive cells to enter S phase. It has also been demonstrated that PS inhibits T_H proliferation and IL-2 synthesis in MLRs. This article aims to analyze the “inhibitory effect” of PS on IL-1 by affecting its synthesis or by inhibiting its specific activity. The authors collected serum from the first or multiple gestational weeks of pregnancy at 8 weeks of gestation, using male donor serum, normal human serum (NHS), as a normal control. Normal donor peripheral blood mononuclear cells (PBMC) as a one-way MLR. Human PBMCs were stimulated with lipopolysaccharide (LPS), and PS and NHS were added respectively and incubated at 37 ° C for 24 hours. Close