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目的了解江苏汉族人群HLA-Bw4及其相应的抑制性杀伤细胞免疫球蛋白样受体(KIR)和激活性KIR与宫颈癌是否存在关联性。方法收集117例宫颈内皮样瘤变3级(CIN3)/宫颈癌患者和124例相匹配的无血缘关系健康女性外周血标本,采用PCR-SSP方法进行HLA-Bw4检测和SYBR GreenⅠreal-time PCR方法进行KIR3DL1和3DS1分型,分析HLA-Bw4及其KIR在2组人群间的差异。结果宫颈癌组与对照组相比,HLA-Bw4、KIR3DL1和3DS1的频率分布无显著性差异(P>0.05)。HLA-Bw4与KIR3DL1和3DS1形成的不同配对组合在病例组与对照组中的频率分布,差异也无统计学意义(P>0.05)。结论江苏汉族人群HLA-Bw4及其KIR3DL1和KIR3DS1可能与宫颈癌的发生无关。
Objective To investigate whether HLA-Bw4 and its corresponding suppressor killer cell immunoglobulin-like receptor (KIR) and activated KIR in Jiangsu Han population are associated with cervical cancer. Methods Serum HLA-Bw4 and SYBR GreenⅠreal-time PCR were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-SSP) in 117 patients with CIN3 / cervical cancer and 124 matched healthy unrelated healthy women. KIR3DL1 and 3DS1 typing were performed to analyze the difference between HLA-Bw4 and KIR in the two groups. Results There was no significant difference in the frequency distribution of HLA-Bw4, KIR3DL1 and 3DS1 between cervical cancer group and control group (P> 0.05). There was also no significant difference in the frequency distribution between HLA-Bw4 and KIR3DL1 and 3DS1 in case group and control group (P> 0.05). Conclusion The HLA-Bw4, KIR3DL1 and KIR3DS1 in Jiangsu Han population may not be related to the occurrence of cervical cancer.