溶剂浓度对提取山高粱中总黄酮含量的影响

• 作 者: 张乐华 于敬海 董陆陆 刘丙义 穆云亭 徐小明
• 机 构: 哈尔滨医科大学化学教研室,哈尔滨医科大学化学教研室,哈尔滨医科大学化学教研室,哈尔滨医科大学化学教研室,哈尔滨医科大学化学教研室,哈尔滨医科大学化学教研室 黑龙江哈尔滨150086,黑龙江哈尔滨150
• 出 处: 哈尔滨医科大学学报
• 发表日期: 2001年05期
• 关键词: 黄酮含量 山高粱 溶剂浓度 分光光度法 植物化学成分 芦丁标准品 蔷薇科植物 曲线回归方程 变异系数 对照品

目的　为从山高粱中提取出较大量的总黄酮寻找提取溶剂的最佳浓度。方法　分别采用 90 %、80 %、70 %、6 0 % 4种浓度的乙醇作为山高粱中黄酮类化合物的提取溶剂 ,进而得到 4种黄酮样品。结果　通过分光光度法测得 4种黄酮样品的总黄酮的含量分别为 1.2 6 %、1.42 %、2 .38%、1.98% ;平均回收率分别为 10 0 .4%、10 1.6 %、10 0 .9%、10 0 .4% ;变异系数分别为 0 .395 %、1.5 8%、0 .45 5 %、0 .946 %。结论　采用乙醇作为山高粱中黄酮类化合物的提取溶剂时 ,乙醇的浓度以 70 %为最佳

Objective To find the optimum concentration of extraction solvent for extracting larger amounts of total flavonoids from sorghum. Methods The concentrations of 40%, 80%, 70%, and 60% of ethanol were used as the extraction solvent for flavonoids from S. sinensis, and then four flavonoids were obtained. Results The contents of total flavonoids in the four flavonoids were 1.26%, 1.4%, 238%, and 1.98%, respectively, measured by spectrophotometry; the average recoveries were 100.4%, 101.6%, and 10%, respectively. The percentages of variance were 0.395%, 1.58%, 0.45% and 0.94%, respectively. Conclusion When ethanol is used as the extraction solvent for flavonoids in sorghum, the ethanol concentration is preferably 70%