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目的 :观察维甲酸 (RA)和地塞米松 (DEX)对骨肉瘤细胞 (HOS)形态和功能分化的诱导作用以及分化后细胞周期与端粒酶活性的变化。方法 :用RA和DEX诱导HOS细胞分化 ,观察分化后瘤细胞的细胞形态和功能的变化。细胞周期用流式细胞仪测定。端粒酶活性用TRAP -银染法检测。结果 :RA和DEX可使体外培养的HOS细胞停留在G2 /M期 ,细胞生长受到明显抑制 ,细胞形态和功能分化成熟 ,碱性磷酸酶 (AP)活性明显升高 ,瘤细胞端粒酶活性随药物作用时间和浓度的增加而明显减弱。结论 :RA和DEX能诱导骨肉瘤细胞形态和功能上的分化 ,端粒酶活性的调节可能发生于细胞周期的S期 ,其活性的下降可作为骨肉瘤细胞分化的指标 ,端粒酶活性下降与细胞分化之间的关系比与细胞周期的关系更为密切
Objective: To observe the effect of retinoic acid (RA) and dexamethasone (DEX) on the differentiation of osteosarcoma cells (HOS) morphology and function and the changes of cell cycle and telomerase activity after differentiation. Methods: The differentiation of HOS cells was induced by RA and DEX. The changes of cell morphology and function after differentiation were observed. The cell cycle was measured by flow cytometry. Telomerase activity was detected by TRAP-silver staining. RESULTS: RA and DEX could keep HOS cells cultured in vitro at G2 / M phase, cell growth was inhibited, cell morphology and function were differentiated and matured, alkaline phosphatase (AP) activity was significantly increased, tumor cell telomerase activity was increased. With the increase of the time and concentration of the drug, it weakened significantly. Conclusion: RA and DEX can induce the differentiation of osteosarcoma cells in morphology and function. The regulation of telomerase activity may occur in the S phase of cell cycle. The decrease of its activity can be used as an indicator of osteosarcoma cell differentiation, and the activity of telomerase is decreased. Relationship with cell differentiation is more closely related to cell cycle