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目的 探讨川芎嗪及硫氮唑酮对照射后ＮＩＨ／３Ｔ３ 成纤维细胞增殖及ｂＦＧＦ表达的影响，为防治放射性肺纤维化的药物研究提供实验依据。方法 采用ＭＴＴ 法测定离体培养的ＮＩＨ／３Ｔ３ 成纤维细胞增殖程度并用免疫细胞化学ＡＢＣ 方法观察ｂＦＧＦ蛋白表达改变。结果 不同组ＭＴＴ法测得光密度值，对照组为０－７１ ±０－２２ ；照射组（Ⅰ） 为１－０３ ±０－２８ ；川芎嗪＋ 照射组为０－４７±０－１５ ；照射组（ Ⅱ） 为１－０５ ±０－２１；硫氮唑酮＋ 照射组为２－０２ ±０－２１；不同组ｂＦＧＦ 蛋白表达平均光密度：对照组为０－０２８±０－００５ ；照射组为０－０４４ ±０－００６ ；川芎嗪＋ 照射组为０－０２９ ±０－００１ ；硫氮唑酮＋ 照射组为０－０３６ ±０－００３。结论 川芎嗪明显抑制γ射线照射后ＮＩＨ／３Ｔ３ 成纤维细胞的增殖及ｂＦＧＦ蛋白表达，硫氮唑酮则不具有这一作用。
Objective To investigate the effects of ligustrazine and thiazolidone on proliferation and bFGF expression of NIH / 3T3 fibroblasts after irradiation, and provide experimental evidence for the prevention and treatment of radiation pulmonary fibrosis. Methods MTT assay was used to determine the proliferation of NIH / 3T3 fibroblasts cultured in vitro. The expression of bFGF protein was observed by immunocytochemical ABC method. Results The optical density values measured by different groups of MTT method were 0-71 ± 0-22 in the control group, 1 ± 0 ± 28 in the irradiation group, 0-47 ± 0-15 in the ligustrazine + irradiation group, The average optical density of bFGF protein in different groups was 0-028 ± 0-005 in the control group; The irradiation group was 0-044 ± 0-006; Ligustrazine + irradiation group was 0-029 ± 0-001; and the group with dithiazole + irradiation group was 0-036 ± 0-003. Conclusion Tetramethylpyrazine can significantly inhibit the proliferation of NIH / 3T3 fibroblasts and the expression of bFGF protein after γ-ray irradiation, while the thiazolidone does not have this effect.