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本文报告~(125)I-血栓素B_2(TXB_2)放射免疫分析及其临床应用。TXB_2抗血清与其它前列腺素的交叉反应均小于0.42％,亲和常数为2.44×10~(10)M~(-1)。冷回收率94.7±7～106.0±13.1％,批内差异6.4％,批间差异11.8％,标准曲线测定范围2.5～160.0Pg/管。人血浆正常值143.5±8.8Pg/mL,尿正常值:229.9±14.4Pg/min。同一份样品用~(125)I和~3H两种放射免疫法测定,二者相关性良好。应用本法对冠心病、心肌梗塞患者及服阿司匹林前后的血浆和尿样品中TXB_2含量进行了探讨。
This article reports ~ (125) I-thromboxane B_2 (TXB_2) radioimmunoassay and its clinical application. The cross - reaction of TXB_2 antiserum with other prostaglandins was less than 0.42%, and the affinity constant was 2.44 × 10 ~ (10) M ~ (-1). Cold recovery 94.7 ± 7 ~ 106.0 ± 13.1%, within-batch differences of 6.4%, inter-batch differences of 11.8%, the standard curve determination range of 2.5 ~ 160.0Pg / tube. Human plasma normal 143.5 ± 8.8Pg / mL, urine normal: 229.9 ± 14.4Pg / min. The same sample with ~ (125) I and ~ 3H radioimmunoassay, the correlation between the two good. The application of this method in coronary heart disease, myocardial infarction patients and before and after taking aspirin plasma and urine samples TXB_2 content were discussed.