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目的 观察AFAP-1 L2下调后对顺铂干预的子宫内膜癌KLE细胞增殖、凋亡及细胞周期的影响,为子宫内膜癌治疗提供新的靶点.方法 以Western blot及qRT-PCR检测不同分化程度子宫内膜癌细胞(KLE,Ishikawa)中AFAP-1L2蛋白及mRNA表达;构建siAFAP-1L2质粒并转染子宫内膜癌细胞,检测转染效率;顺铂干预siNegative Control细胞为对照组(DDP组),对siAFAP-1 L2干扰细胞(siAFAP-1L2+DDP组)给予不同浓度顺铂干预或同一浓度不同培养时间下以MTT法观察细胞存活率,流式细胞术观察细胞凋亡率,PI法观察细胞周期.结果 AFAP-1L2蛋白及mRNA在低分化KLE细胞株中表达水平明显高于高分化Ishikawa细胞株.AFAP-1L2下调后能够增加KLE细胞对DDP化疗敏感度,并具有剂量依赖性及时间依赖性.下调AFAP-1 L2表达能够促进DDP干预的KLE细胞凋亡,使更多细胞生长停滞.结论 siAFAP-1L2沉默可增强顺铂对子宫内膜癌细胞的杀灭作用,为子宫内膜癌治疗研究的候选靶点.“,”Objective To observe the effect of AFAP-1L2 down-regulating on the proliferation,apoptosis and cell cycle of endometrial cancer cells (KLE,Ishikawa) interfered by cisplatin.Methods Western blot and (qRT)-PCR were used to detect AFAP-1L2 protein and mRNA expressions in different differentiation endometrial cancer cells.The siAFAP-1L2 plasmid was constructed and transfected into endometrial cancer cells.The cell viability was observed by MTT assay in different DDP concentration or different culture time in siAFAP-1L2 interfering cells(siAFAP-1L2+DDP group).Cell apoptosis and cycle were detected by flow cytometry and PI method.Results The expressed level of AFAP-1 L2 protein and mRNA was higher in low differentiation KLE cells than in high differentiation Ishikawa cells.AFAP-1L2 silence attenuated the sensitivity of KLE cells to DDP chemotherapy with concentration dependent and time dependent,attenuated KLE cell apoptosis dealt with DDP and more cells growing stop.Conclusion siAFAP-1L2 interfering may attenuate the killing effect of cisplatin to endometrial cancer cells and may become a candidate target for endometrial cancer therapeutic research.