论文部分内容阅读
目的 探讨增生性T细胞 (proliferatingTcell,PTC)在扁桃体和B细胞来源的淋巴瘤中的分布及意义。方法 应用免疫组化双标记方法检测PTC ,应用Giemsa染色检测胃MALT淋巴瘤中幽门螺杆菌 (Hp)感染情况 ,用细胞培养检测胃MALT淋巴瘤中肿瘤细胞与Hp及T细胞的关系。结果在扁桃体的正常滤泡内与滤泡性淋巴瘤的肿瘤性滤泡内几乎找不到CD3与MIB1双阳性的PTC ,PTC主要分布在滤泡外与T区。CD3表达CD2 5呈相同分布情况。低度恶性胃粘膜相关淋巴组织型淋巴瘤与Hp的感染有关。细胞培养祛除T细胞后肿瘤细胞死亡。在弥漫型B细胞淋巴瘤中 ,PTC依恶性程度呈梯度表达。结论 在扁桃体的正常滤泡内与滤泡性淋巴瘤的肿瘤性滤泡内T细胞处于G0 期 ,胃MALT淋巴瘤中肿瘤性B细胞的增生可能依赖于Hp特异性的T细胞。肿瘤细胞向高度恶性转化的过程中与T细胞活性增强有关 ,T细胞表达MIB1可能与患者预后有关
Objective To investigate the distribution and significance of proliferating T cells (PTCs) in tonsil and B cell-derived lymphomas. Methods PTC was detected by immunohistochemistry double labeling method. Helicobacter pylori (Hp) infection was detected by Giemsa staining in gastric MALT lymphoma. The relationship between Hp and T cells in gastric MALT lymphoma was detected by cell culture. Results In the normal follicles of tonsils and tumor follicles of follicular lymphomas, double positive CD3 and MIB1 PTC were not found. PTC was mainly located in the outer follicles and T area. CD3 expression was the same distribution of CD25. Low-grade gastric mucosa-associated lymphoid tissue type lymphoma is associated with Hp infection. The cell death occurred after cell culture cultured T cells. In diffuse B-cell lymphomas, PTCs are expressed in gradients depending on the degree of malignancy. Conclusion In the normal follicles of the tonsils and follicular lymphomas, the T-cells in the follicular follicles are in the G0 phase. The hyperplasia of the neoplastic B cells in gastric MALT lymphomas may depend on H-specific T cells. Increased T-cell activity in the process of high malignant transformation of tumor cells. The expression of MIB1 in T cells may be related to the prognosis of patients.