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为了解我市卡介苗纳入“冷链”运转后的情况,于1986年夏天(气温27℃～33℃)对成都生研所批号“860724”液体皮内卡介苗,进行了活菌数及接种效果监测,现报告如下。材料与方法一、选点在西城区“冷链”点,随机抽样10个,作为监测点。二、菌苗抽样从菌苗出所经市、区防疫站运输、储存、转发到接种点的几个“冷链”运转环节,随机取样作活菌数监测实验。三、活菌计数方法采取常规活菌计数法,将培养结果按WHO的计算公式计算活菌数。材料、技术均由成都生研所提供。四、接种效果观察以监测点的初种婴儿为观察对象,按接种时间分为新鲜菌苗与非新鲜菌苗两组。接种后12周,由市结防院用成都生研所“860912”批旧结素5 Tu,作阳转率复查及接种反应观察。监测结果一、活菌数实验结果 1.菌苗出所时活菌数为
In order to understand the situation of BCG in our city after the operation of “cold chain”, we monitored viable count and inoculation effect of “860724” liquid intradermal BCG vaccine of Chengdu Institute of Health in the summer of 1986 (temperature 27 ℃ ~ 33 ℃) The report is as follows. Materials and methods First, the selection point in the “cold chain” point in Xicheng District, a random sample of 10, as a monitoring point. Second, the vaccine sample from the vaccine out of the city, district epidemic prevention station transport, storage, forwarding to the point of inoculation of several “cold chain” part of the operation, random sampling for viable count monitoring experiments. Third, the viable count method to take conventional viable count method, the culture results according to WHO’s formula to calculate viable count. Materials, technology provided by Chengdu Institute of Health. Fourth, the observation of vaccination effect To the monitoring point of the first infants as the observation object, according to the vaccination time is divided into two groups of fresh and non-new bacterins vaccine. Twelve weeks after inoculation, the city knot-guard institute approved Chengdu Tuen Research Institute “860912” batch of old knot 5 Tu for review of the positive conversion rate and inoculation reaction observation. Monitoring results First, the number of live bacteria test results 1. Bacteria out of the viable count