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一般中性磷化合物(磷酸三烷基酯、烷基瞵酸二烷基酯、二烷基瞵酸烷基酯、氧化三烷基瞵、亚磷酸二烷基酯及亚磷酸三烷基酯)皆能借乙酰化滤纸为固定相,进行纸上层析分离鉴定.化合物结构类型、烷基碳原子数目、展开剂性质对纸上层析比移值均有显著影响.化合物的极性及分子量是决定纸上层析行为的主要因素.滤纸的乙酰值对层析行为亦有影响.所提供的 Hanes-Isherwood 试剂显色改良法,具有操作简捷、色斑清晰及色谱易于保存的优点.对一般中性磷化合物的显色灵敏度为1微克.中性磷化合物的纸上层析色斑面积与其含量有较好的正比关系,借面积计算法可进行纸上层析的定量测定,实验误差为±3%.
General Neutral Phosphorus Compounds (trialkyl phosphates, dialkyl alkyl dialkyls, dialkyl dialkyl alkanoates, trialkyl silicones, dialkyl phosphites, and trialkyl phosphites) Acetylation filter paper can be used as stationary phase for chromatographic separation and identification.Compound structure type, number of alkyl carbon atoms, the nature of the developer on the chromatographic ratio of paper have a significant impact on the polarity of the compound and molecular weight Is the main factor that determines the behavior of paper chromatography.The acetyl value of filter paper also has an impact on the chromatographic behavior.The provided Hanes-Isherwood reagent color development method has the advantages of simple operation, clear stain and easy chromatographic storage. The general color neutral phosphor neutral sensitivity of 1 microgram neutral phosphorus compounds paper chromatography stain area and its content has a good positive relationship, by area calculation method for quantitative determination of paper chromatography, experimental error Is ± 3%.